Fig. 5. Ang II induces podocyte apoptosis. Podocyte apoptosis is induced by Ang II in both time- and concentration-dependent manners as determined using FACS (A, B) and TUNEL assay (C, D). Nuclei are colored in blue by DAPI in the TUNEL assay. Data are expressed as mean ± SD (n = 3). ^*P<0.05 and ^**P<0.01 versus control (without Ang II at 0 hour). ^＃P<0.05 and ^＃＃P<0.01 versus control at each incubation time. Magnification, ×200; Bar = 100 μm.